Risk Associated with NAD (P) H: Quinone Oxidoreductase I (NQOI) C609T Polymorphism for Cigarette Smoke Induced Cardiovascular Disease (CVD): A Study on Male Current Smokers from Eastern India

NAD(P)H: quinone oxidoreductase 1 (NQO1) is a well-known enzyme for its protective role against cigarette smoke (CS) derived harmful quinones such as p-benzoquinone which major causative agent of CS-related cardiovascular disease (CVD). NQO1 C609T single nucleotide polymorphism has severe consequence on function and availability. We propose that the presence variant allele this exposes smokers towards greater risk developing CVD. For verification hypothesis we perform genotype analysis SNP in two groups male current (smokers with CVD, n=100 without n=200) by PCR-RFLP method. Genotype distributions both were Hardy Weinberg equilibrium. The wild type (p) (q) frequencies different these (in CVD: p=67.3%, q=32.7%; p=59%, q=41%). significantly between smoker CC=43.5%, CT=47.5% TT=9%; CC=32%, CT=54% TT=14%). Cochran-Armitage-linear Trend Test indicated ‘proportions CVD’ have linear trend number alleles (CA χ2=4.329, p<0.05). variation distribution was analysed genetic test model. Significant observed additive model (p<0.05). odds ratios (OR) heterozygous (CT) homozygous (TT) than one (for CT: OR=1.55, 95%CI: 0.91-2.61; TT: OR=2.11, 0.94-4.74).Thus, study strongly associated CS- related CVD smokers.